Time variation of adipose tissue refractive index under photodynamic treatment: in vitro study using OCT

Irina Yu. Yanina; Natalia A. Trunina; Valery V. Tuchin

doi:10.1117/12.915485

9 February 2012 Time variation of adipose tissue refractive index under photodynamic treatment: in vitro study using OCT

Irina Yu. Yanina, Natalia A. Trunina, Valery V. Tuchin

Author Affiliations +

Proceedings Volume 8222, Dynamics and Fluctuations in Biomedical Photonics IX; 82221G (2012) https://doi.org/10.1117/12.915485
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Time variation of the adipose tissue refractive index under photodynamic treatment was studied using OCT. Fat tissue slices 200-500 μm thick were used in in vitro experiments. To stain the fat tissue we used water-ethanol solutions of indocyanine green (ICG) and brilliant green (BG) with the concentration 1 mg/ml and 6 mg/ml, respectively. The CW laser diode (VD-VII DPSS, 808 nm) and the dental diode irradiator Ultra Lume Led 5 (442 and 597 nm) were used for irradiation of tissue slices. The irradiation time was 5 min for the laser and 15 min for the diode lamp. The experiments were carried out at room temperature. It was discovered the immersion optical clearing of fat tissue slices due to fat cell lipolysis under photodynamic treatment. Released cell content works as an immersion agent, thus the relative refractive index of tissue scatterers decreasing with the time elapsed after the treatment. These data support the hypothesis that photodynamic treatment induces fat cell lipolysis for some period after treatment.

Citation Download Citation

Irina Yu. Yanina, Natalia A. Trunina, and Valery V. Tuchin "Time variation of adipose tissue refractive index under photodynamic treatment: in vitro study using OCT", Proc. SPIE 8222, Dynamics and Fluctuations in Biomedical Photonics IX, 82221G (9 February 2012); https://doi.org/10.1117/12.915485

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