Random access multiphoton (RAMP) microscopy for investigation of cerebral blood flow regulation mechanisms

Daniel J. Christensen; Maiken Nedergaard

doi:10.1117/12.907141

9 February 2012 Random access multiphoton (RAMP) microscopy for investigation of cerebral blood flow regulation mechanisms

Daniel J. Christensen, Maiken Nedergaard

Author Affiliations +

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 822633 (2012) https://doi.org/10.1117/12.907141
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The processes by which blood flow is regulated at the capillary network level in the brain has been a source of continual debate. It is generally accepted that cerebral blood flow regulation occurs primarily at the arteriolar level. It has been recently suggested, however, that the capillary network is likewise under dynamic regulation. The exact mechanisms of capillary regulation remain unknown. Previously, the limiting factor in determining how the cerebrovascular network is regulated has been the speed at which multiphoton images of large (~200μm²) capillary and arteriole systems can be acquired. Conventional laser scanning microscopy systems are temporally limited in two dimensions. We have developed a Random Access Multiphoton (RAMP) microscope, which operates on the principles of Acousto-optic beam scanning and therefore has no moving parts, specifically for the purpose of imaging blood flow virtually simultaneously throughout the capillary network. We demonstrate the ability to survey blood flow simultaneously in 100 capillaries.

Citation Download Citation

Daniel J. Christensen and Maiken Nedergaard "Random access multiphoton (RAMP) microscopy for investigation of cerebral blood flow regulation mechanisms", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822633 (9 February 2012); https://doi.org/10.1117/12.907141

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