Fluorescence enhancements and spectral modifications near the cut-off frequency of plasmonic structure

Kareem Elsayad; Alexander Urich; Maria Nemethova; John V. Small; Karl Unterrainer; Katrin G. Heinze

doi:10.1117/12.909084

6 February 2012 Fluorescence enhancements and spectral modifications near the cut-off frequency of plasmonic structure

Kareem Elsayad, Alexander Urich, Maria Nemethova, John V. Small, Karl Unterrainer, Katrin G. Heinze

Proceedings Volume 8234, Plasmonics in Biology and Medicine IX; 82340W (2012) https://doi.org/10.1117/12.909084
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The effect of a population of fluorophores coupling to weakly bound surface plasmons in dielectric/metal/dielectric structures is investigated for the purpose of fluorescence enhancement near interfaces and live cell fluorescence surface imaging. We show theoretically and experimentally that for sufficient fluorophore concentrations near such SPP supporting structures significant enhancements in the radiative emission intensity can be observed, with a spectral modification that can be correlated to the average separation of the fluorophores from the substrate. We will discuss the theory behind the effect and some experimental results on imaging labeled proteins in the focal adhesion sites of cells.

Citation Download Citation

Kareem Elsayad, Alexander Urich, Maria Nemethova, John V. Small, Karl Unterrainer, and Katrin G. Heinze "Fluorescence enhancements and spectral modifications near the cut-off frequency of plasmonic structure", Proc. SPIE 8234, Plasmonics in Biology and Medicine IX, 82340W (6 February 2012); https://doi.org/10.1117/12.909084

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