Amplification-free detection of miRNA via an ECL chips system

Weipeng Liu; Xiaoming Zhou

doi:10.1117/12.999861

11 December 2012 Amplification-free detection of miRNA via an ECL chips system

Weipeng Liu, Xiaoming Zhou

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 855336 (2012) https://doi.org/10.1117/12.999861
Event: Photonics Asia, 2012, Beijing, China

Abstract

MicroRNAs (miRNAs) play pivotal roles in many fundamental aspects of life, such as tissue differentiation, metabolic modulation and cell proliferation control. As miRNA has the characteristics of small size, easy degradation and low abundance, a simple, rapid and specificity method to measure the concentration of miRNA is required. Herein, we present an Electrochemiluminescent Chips system for rapid miRNA detection based on the base stacking hybridization and magnetic beads (MB) enrichment technology. In this design, integrating the microfluidic system with Electrochemiluminescent detection makes it easy to assemble the multiple assay steps (e.g., sample preparation, incubation, washing and detection) and let the device convenient to carry. This method is very fast, we can detect miRNA in 19 min with a 10 uL sample volume. Moreover, in this experiment the detection limit was 1 fmol of the pure synthetic miRNA, and the linear range was from 5 fmol to 1 pmol. It also exhibited excellent selectivity over a series of interference miRNA, which display a high level of expression in cancer cell. These results suggest that this method might have potential for cancer diagnosis at the point of care.

Citation Download Citation

Weipeng Liu and Xiaoming Zhou "Amplification-free detection of miRNA via an ECL chips system", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855336 (11 December 2012); https://doi.org/10.1117/12.999861

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