3D micron-scale imaging of the cortical bone canal network in human osteogenesis imperfecta (OI)

John R. Jameson; Carolyne I. Albert; Bjoern Busse; Peter A. Smith; Gerald F. Harris

doi:10.1117/12.2007209

29 March 2013 3D micron-scale imaging of the cortical bone canal network in human osteogenesis imperfecta (OI)

John R. Jameson, Carolyne I. Albert, Bjoern Busse, Peter A. Smith, Gerald F. Harris

Proceedings Volume 8672, Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging; 86721L (2013) https://doi.org/10.1117/12.2007209
Event: SPIE Medical Imaging, 2013, Lake Buena Vista (Orlando Area), Florida, United States

Abstract

Osteogenesis imperfecta (OI) is a genetic disorder leading to increased bone fragility. Recent work has shown that the hierarchical structure of bone plays an important role in determining its mechanical properties and resistance to fracture. The current study represents one of the first attempts to characterize the 3D structure and composition of cortical bone in OI at the micron-scale. A total of 26 pediatric bone fragments from 18 individuals were collected during autopsy (N_c=5) or routing orthopaedic procedures (N_OI=13) and imaged by microtomography with a synchrotron light source (SRμCT) for several microstructural parameters including cortical porosity (Ca.V/TV), canal surface to tissue volume (Ca.S/TV), canal diameter (Ca.Dm), canal separation (Ca.Sp), canal connectivity density (Ca.ConnD), and volumetric tissue mineral density (TMD). Results indicated significant differences in all imaging parameters between pediatric controls and OI tissue, with OI bone showing drastically increased cortical porosity, canal diameter, and connectivity. Preliminary mechanical testing revealed a possible link between cortical porosity and strength. Together these results suggest that the pore network in OI contributes greatly to its reduced mechanical properties.

Citation Download Citation

John R. Jameson, Carolyne I. Albert, Bjoern Busse, Peter A. Smith, and Gerald F. Harris "3D micron-scale imaging of the cortical bone canal network in human osteogenesis imperfecta (OI)", Proc. SPIE 8672, Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging, 86721L (29 March 2013); https://doi.org/10.1117/12.2007209

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available