Enzyme-linked monoclonal antibody microstructured optical fiber monitor

Julie DellAntonio; Nicole Giambrone; Francis Anuszewski; Rosalind Wynne; William Kelly

doi:10.1117/12.2009509

23 April 2013 Enzyme-linked monoclonal antibody microstructured optical fiber monitor

Julie DellAntonio, Nicole Giambrone, Francis Anuszewski, Rosalind Wynne, William Kelly

Proceedings Volume 8693, Smart Sensor Phenomena, Technology, Networks, and Systems Integration 2013; 86930U (2013) https://doi.org/10.1117/12.2009509
Event: SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring, 2013, San Diego, California, United States

Abstract

A solid-core photonic crystal fiber (SC-PCF) with a microstructured all-silica steering wheel lattice containing a 3 μm diameter core is used to identify dye-free enzyme-linked monoclonal antibodies (mAbs) of very small volumes <0.1uL for concentrations ranging from 0.1mg/mL to 10^-12 mg/mL. The samples used in this study are mAbs, which are useful in detecting the presence of the botulism toxin. Human IgG antibodies (Southern Biotech Inc.) served as the “coating” antibody to detect an enzyme-linked mAb (Southern Biotech Inc.) - the “antigen”. The spectra for mAb samples linked with enzymes were compared to control samples without enzymes and conventional ELISA measurements. The SC-PCF at lengths 1m and 0.5m were filled with ~ 0.008 μL of each sample was coupled with a broadband LED light source with emission peaks in the 1280-1700 nm range. Absorption characteristics associated with these mAbs were measured using a standard optical spectrum analyzer. Spectra were collected for control antibody samples, enzyme-linked antibody and a third sample of a mixture of both antibodies. The measured spectra for the samples with varied concentrations were consistent with conventional ELISA measurements. Limitations due to bend loss were also investigated. A compact, real-time sensor based on this dye-free technology has the potential to benefit water quality monitoring, drug manufacturing and food quality control.

Citation Download Citation

Julie DellAntonio, Nicole Giambrone, Francis Anuszewski, Rosalind Wynne, and William Kelly "Enzyme-linked monoclonal antibody microstructured optical fiber monitor", Proc. SPIE 8693, Smart Sensor Phenomena, Technology, Networks, and Systems Integration 2013, 86930U (23 April 2013); https://doi.org/10.1117/12.2009509

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Sensors

Absorption

Structured optical fibers

Molecules

Single mode fibers

Monoclonal antibodies

Light emitting diodes

Show All Keywords

Keywords/Phrases

Search In:

Publication Years