Technique of laser confocal and Raman spectroscopy for living cell analysis

Xiaochen Meng; Lianqing Zhu

doi:10.1117/12.2035491

10 October 2013 Technique of laser confocal and Raman spectroscopy for living cell analysis

Xiaochen Meng, Lianqing Zhu

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Proceedings Volume 8916, Sixth International Symposium on Precision Mechanical Measurements; 891612 (2013) https://doi.org/10.1117/12.2035491
Event: Sixth International Symposium on Precision Mechanical Measurements, 2013, Guiyang, China

Abstract

Because of the shortcomings of the main methods used to analysis single cell, the need of single living cell analysis with no damage, unmarked and in situ dynamic multi-parameter measurement is urgent in the life sciences and biomedical advanced research field. And the method of for living cells analysis is proposed. The spectral pretreatment technology of living cell is the key work of laser confocal Raman spectroscopy. To study the spectrum processing methods for Raman spectrum on single living cell and develop the pre-process techniques to enhance the signal-to-noise ratio, sensitivity, and decrease the influence of fluorescence, elimination the cosmic rays was used to improve the spectrum. The classification, average and filtration of spectrum were applied to enhance signal-to-noise ratio. The fluorescence was depressed for quantity analysis or utilized for analysis by comparing the background and the spectrum. The results show that the proposed technique for laser confocal Raman spectrum of single cell can perform the sensitive and weak intensity peaks and reflect the information of molecules structures very well.

Citation Download Citation

Xiaochen Meng and Lianqing Zhu "Technique of laser confocal and Raman spectroscopy for living cell analysis", Proc. SPIE 8916, Sixth International Symposium on Precision Mechanical Measurements, 891612 (10 October 2013); https://doi.org/10.1117/12.2035491

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KEYWORDS

Raman spectroscopy

Luminescence

Confocal microscopy

Analytical research

Biological research

Optical filters

Smoothing

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