In vivo Raman spectroscopy of cervix cancers

S. Rubina; Priyanka Sathe; Tapas Kumar Dora; Supriya Chopra; Amita Maheshwari; C. Murali Krishna

doi:10.1117/12.2033937

17 March 2014 In vivo Raman spectroscopy of cervix cancers

S. Rubina, Priyanka Sathe, Tapas Kumar Dora, Supriya Chopra, Amita Maheshwari, C. Murali Krishna

Proceedings Volume 8940, Optical Biopsy XII; 89400E (2014) https://doi.org/10.1117/12.2033937
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Cervix-cancer is the third most common female cancer worldwide. It is the leading cancer among Indian females with more than million new diagnosed cases and 50% mortality, annually. The high mortality rates can be attributed to late diagnosis. Efficacy of Raman spectroscopy in classification of normal and pathological conditions in cervix cancers on diverse populations has already been demonstrated. Our earlier ex vivo studies have shown the feasibility of classifying normal and cancer cervix tissues as well as responders/non-responders to Concurrent chemoradiotherapy (CCRT). The present study was carried out to explore feasibility of in vivo Raman spectroscopic methods in classifying normal and cancerous conditions in Indian population. A total of 182 normal and 132 tumor in vivo Raman spectra, from 63 subjects, were recorded using a fiberoptic probe coupled HE-785 spectrometer, under clinical supervision. Spectra were acquired for 5 s and averaged over 3 times at 80 mW laser power. Spectra of normal conditions suggest strong collagenous features and abundance of non-collagenous proteins and DNA in case of tumors. Preprocessed spectra were subjected to Principal Component-Linear Discrimination Analysis (PCLDA) followed by leave-one-out-cross-validation. Classification efficiency of ~96.7% and 100% for normal and cancerous conditions respectively, were observed. Findings of the study corroborates earlier studies and suggest applicability of Raman spectroscopic methods in combination with appropriate multivariate tool for objective, noninvasive and rapid diagnosis of cervical cancers in Indian population. In view of encouraging results, extensive validation studies will be undertaken to confirm the findings.

Citation Download Citation

S. Rubina, Priyanka Sathe, Tapas Kumar Dora, Supriya Chopra, Amita Maheshwari, and C. Murali Krishna "In vivo Raman spectroscopy of cervix cancers", Proc. SPIE 8940, Optical Biopsy XII, 89400E (17 March 2014); https://doi.org/10.1117/12.2033937

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