Optical signature of multicellular tumor spheroid using index-mismatch-induced spherical aberrations

G. Le Corre; P. Weiss; B. Ducommun; C. Lorenzo

doi:10.1117/12.2041339

26 February 2014 Optical signature of multicellular tumor spheroid using index-mismatch-induced spherical aberrations

G. Le Corre, P. Weiss, B. Ducommun, C. Lorenzo

Author Affiliations +

Proceedings Volume 8941, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications; 894111 (2014) https://doi.org/10.1117/12.2041339
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The development of new cancer treatments and the early prediction of their therapeutic potential are often made difficult by the lack of predictive pharmacological models. The 3D multicellular tumor spheroid (MCTS) model offers a level of complexity that recapitulates the three-dimensional organization of a tumor and appears to be fairly predictive of therapeutic efficiency. The use of spheroids in large-scale automated screening was recently reported to link the power of a high throughput analysis to the predictability of a 3D cell model. The spheroid has a radial symmetry; this simple geometry allows establishing a direct correlation between structure and function. The outmost layers of MCTS are composed of proliferating cells and form structurally uniform domain with an approximate thickness of 100 microns. The innermost layers are composed of quiescent cells. Finally, cells in the center of the spheroid can form a necrotic core. This latest region is structurally heterogeneous and is poorly characterized. These features make the spheroid a model of choice and a paradigm to study the optical properties of various epithelial tissues. In this study, we used an in-vitro optical technique for label-free characterization of multicellular systems based on the index- mismatch induced spherical aberrations. We achieve to monitor and characterize the optical properties of MCTS. This new and original approach might be of major interest for the development of innovative screening strategies dedicated to the identification of anticancer drugs.

Citation Download Citation

G. Le Corre, P. Weiss, B. Ducommun, and C. Lorenzo "Optical signature of multicellular tumor spheroid using index-mismatch-induced spherical aberrations", Proc. SPIE 8941, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications, 894111 (26 February 2014); https://doi.org/10.1117/12.2041339

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

3D modeling

Monochromatic aberrations

Tumors

Refractive index

Optical properties

Cancer

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years