Quad stereo-microscopy

Rebecca F. Hay; Graham M. Gibson; Michael P. Lee; Miles J. Padgett; David B. Phillips

doi:10.1117/12.2061913

16 September 2014 Quad stereo-microscopy

Rebecca F. Hay, Graham M. Gibson, Michael P. Lee, Miles J. Padgett, David B. Phillips

Proceedings Volume 9164, Optical Trapping and Optical Micromanipulation XI; 916410 (2014) https://doi.org/10.1117/12.2061913
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

Stereo-microscopy is a technique that enables a sample to be imaged from two directions simultaneously, allowing the tracking of microscopic objects in three dimensions. This is achieved by illuminating the sample from different directions, each illumination direction producing an individual image. These images are superimposed in the image plane but can be easily separated using a diffractive optical element in the Fourier plane of the imaging arm. Therefore this enables 3-dimensional coordinates to be reconstructed using simple 2-dimensional image tracking and parallax. This is a powerful technique when combined with holographic optical tweezers (HOT), where multiple objects can be trapped and tracked simultaneously in three dimensions. In this work, we extend this concept to four different illumination directions: quad stereo-microscopy. This allows us to measure the accuracy of tracking in three dimensions, and to optimise the system.

Citation Download Citation

Rebecca F. Hay, Graham M. Gibson, Michael P. Lee, Miles J. Padgett, and David B. Phillips "Quad stereo-microscopy", Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916410 (16 September 2014); https://doi.org/10.1117/12.2061913

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KEYWORDS

Cameras

Fiber optic illuminators

Optical tracking

Spatial light modulators

3D metrology

3D image processing

3D image reconstruction

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