Cell-based optical assay for amyloid β-induced neuronal cell dysfunction using femtosecond-pulsed laser

Seunghee Lee; Jonghee Yoon; Chulhee Choi

doi:10.1117/12.2077416

10 March 2015 Cell-based optical assay for amyloid β-induced neuronal cell dysfunction using femtosecond-pulsed laser

Seunghee Lee, Jonghee Yoon, Chulhee Choi

Author Affiliations +

Proceedings Volume 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II; 93051Y (2015) https://doi.org/10.1117/12.2077416
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Amyloid β-protein (Aβ) is known as a key molecule related to the pathogenesis of Alzheimer’s disease (AD). Over time, the amyloid cascade disrupts essential function of mitochondria including Ca²⁺ homeostasis and reactive oxygen species (ROS) regulation, and eventually leads to neuronal cell death. However, there have been no methods that analyze and measure neuronal dysfuction in pathologic conditions quantitatively. Here, we suggest a cell-based optical assay to investigate neuronal function in AD using femtosecond-pulsed laser stimulation. We observed that laser stimulation on primary rat hippocampal neurons for a few microseconds induced intracellular Ca²⁺ level increases or produced intracellular ROS which was a primary cause of neuronal cell death depending on delivered energy. Although Aβ treatment alone had little effect on the neuronal morphologies and networks in a few hours, Aβ-treated neurons showed delayed Ca²⁺ increasing pattern and were more vulnerable to laser-induced cell death compared to normal neurons. Our results collectively indicate that femtosecond laser stimulation can be a useful tool to study neuronal dysfuction related to AD pathologies. We anticipate this optical method to enable studies in the early progression of neuronal impairments and the quantitative evaluation of drug effects on neurons in neurodegenerative diseases, including AD and Parkinson’s disease in a preclinical study.

Citation Download Citation

Seunghee Lee, Jonghee Yoon, and Chulhee Choi "Cell-based optical assay for amyloid β-induced neuronal cell dysfunction using femtosecond-pulsed laser", Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 93051Y (10 March 2015); https://doi.org/10.1117/12.2077416

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