Femtosecond laser collagen cross-linking without traditional photosensitizers

Yizang Guo; Chao Wang; Nicola Celi; Sinisa Vukelic

doi:10.1117/12.2079608

5 March 2015 Femtosecond laser collagen cross-linking without traditional photosensitizers

Yizang Guo, Chao Wang, Nicola Celi, Sinisa Vukelic

Author Affiliations +

Proceedings Volume 9321, Optical Interactions with Tissue and Cells XXVI; 932103 (2015) https://doi.org/10.1117/12.2079608
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Collagen cross-linking in cornea has the capability of enhancing its mechanical properties and thereby providing an alternative treatment for eye diseases such as keratoconus. Currently, riboflavin assisted UVA light irradiation is a method of choice for cross-link induction in eyes. However, ultrafast pulsed laser interactions may be a powerful alternative enabling in-depth treatment while simultaneously diminishing harmful side effects such as, keratocyte apoptosis. In this study, femtosecond laser is utilized for treatment of bovine cornea slices. It is hypothesized that nonlinear absorption of femtosecond laser pulses plays a major role in the maturation of immature cross-links and the promotion of their growth. Targeted irradiation with tightly focused laser pulses allows for the absence of a photosensitizing agent. Inflation test was conducted on half treated porcine cornea to identify the changes of mechanical properties due to laser treatment. Raman spectroscopy was utilized to study subtle changes in the chemical composition of treated cornea. The effects of treatment are analyzed by observing shifts in Amide I and Amide III bands, which suggest deformation of the collagen structure in cornea due to presence of newly formed cross-links.

Citation Download Citation

Yizang Guo, Chao Wang, Nicola Celi, and Sinisa Vukelic "Femtosecond laser collagen cross-linking without traditional photosensitizers", Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 932103 (5 March 2015); https://doi.org/10.1117/12.2079608

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