The use of microfluidics and dielectrophoresis for separation, concentration, and identification of bacteria

Cynthia Hanson; Michael Sieverts; Karen Tew; Annelise Dykes; Michaela Salisbury; Elizabeth Vargis

doi:10.1117/12.2213593

21 March 2016 The use of microfluidics and dielectrophoresis for separation, concentration, and identification of bacteria

Cynthia Hanson, Michael Sieverts, Karen Tew, Annelise Dykes, Michaela Salisbury, Elizabeth Vargis

Author Affiliations +

Proceedings Volume 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV; 97050E (2016) https://doi.org/10.1117/12.2213593
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Traditional bacterial identification methods take one to two days to complete, relying on large bacteria colonies for visual identification. In order to decrease this analysis time in a cost-effective manner, a method to sort and concentrate bacteria based on the bacteria’s characteristics itself is needed. One example of such a method is dielectrophoresis, which has been used by researchers to separate bacteria from sample debris and sort bacteria according to species. This work presents variations in which dielectrophoresis can be performed and their associated drawbacks and benefits specifically to bacterial identification. In addition, a potential microfluidic design will be discussed.

Conference Presentation

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Cynthia Hanson, Michael Sieverts, Karen Tew, Annelise Dykes, Michaela Salisbury, and Elizabeth Vargis "The use of microfluidics and dielectrophoresis for separation, concentration, and identification of bacteria", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 97050E (21 March 2016); https://doi.org/10.1117/12.2213593

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