Light pattern preservation in rodent's cortical tissue during optogenetic neuro-stimulation
(Conference Presentation)

Mehdi Azimipour; Farid Atry; Ramin Pashaie

doi:10.1117/12.2213201

26 April 2016 Light pattern preservation in rodent's cortical tissue during optogenetic neuro-stimulation (Conference Presentation)

Mehdi Azimipour, Farid Atry, Ramin Pashaie

Author Affiliations +

Proceedings Volume 9706, Optical Interactions with Tissue and Cells XXVII; 970610 (2016) https://doi.org/10.1117/12.2213201
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Optogenetics provides a tool for modulating activity of specific cell types by light pulses. Different light delivery mechanisms such as single optical fiber implanted on a skull or patterned illumination can be employed to direct light to a target area. For a highly scattering medium such as brain tissue, light distribution significantly depends on the scattering parameters of the tissue as well as the inherent inhomogeneity of the specimen. For in vivo studies, blood vessels which have considerable absorption coefficient in the visible spectrum play a major role in producing such inhomogeneity. Therefore, detailed information about brain optical properties and network of blood vessels which was ignored in previous studies is necessary to accurately predict light distribution and designing light delivery mechanism during optogenetic experiments to achieve the desired optical stimulation. In this paper, light pattern preservation while considering the impact of blood vessels is investigated in a rat cortex. First, the typical optical properties of rat cortical tissue were extracted by employing double integrated sphere technique, and then, optical coherence tomography was employed to obtain structure of blood vessels on the cortex. By combining the extracted optical properties and the network of blood vessels, a three-dimensional model of a rat cortical tissue was developed. Then, a Monte Carlo simulation code was used to predict light distribution in this model for different light source configurations and wavelengths. The results confirm that presence of vessels can significantly impact the light pattern in the tissue and affect the practical depth of penetration.

Conference Presentation

Citation Download Citation

Mehdi Azimipour, Farid Atry, and Ramin Pashaie "Light pattern preservation in rodent's cortical tissue during optogenetic neuro-stimulation (Conference Presentation)", Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 970610 (26 April 2016); https://doi.org/10.1117/12.2213201

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Tissue optics

Blood vessels

Optogenetics

3D modeling

Optical properties

Brain

Light scattering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years