Non-linear photoacoustic and fluorescence microscopy using a modulated laser diode

G. Langer; T. Berer

doi:10.1117/12.2213027

15 March 2016 Non-linear photoacoustic and fluorescence microscopy using a modulated laser diode

G. Langer, T. Berer

Author Affiliations +

Proceedings Volume 9708, Photons Plus Ultrasound: Imaging and Sensing 2016; 97082K (2016) https://doi.org/10.1117/12.2213027
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present simultaneous photoacoustic and fluorescence microscopy using a modulated laser diode. Photoacoustic waves and modulated fluorescence are generated by using a laser diode with a wavelength of 405nm at modulation frequencies in the Megahertz range. Additionally, a continuous wave offset radiation can be superposed. The excitation light is focused to the sample using a high NA objective. Luminescence is collected by the same objective in a confocal configuration and measured by an avalanche photo diode. Photoacoustic waves are recorded on the opposite site of the sample using a hydrophone. Acoustic and luminescence signals are recorded using a lock-in technique. We present photoacoustic imaging on red blood cells and multimodal imaging on fluorescent microspheres. Nonlinear photoacoustic and luminescence effects were introduced with the help of a superposed continuous wave laser.

Conference Presentation

Citation Download Citation

G. Langer and T. Berer "Non-linear photoacoustic and fluorescence microscopy using a modulated laser diode", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97082K (15 March 2016); https://doi.org/10.1117/12.2213027

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