Spatially targeted non-viral optical delivery of opsin-encoding genes into primate retina

Biraj Mahato; Sanghoon Kim; Houssam Al-Saad; Michael Carlson; Subrata Batabyal; Adnan Dibas; Samarendra K. Mohanty

doi:10.1117/12.2615466

7 March 2022 Spatially targeted non-viral optical delivery of opsin-encoding genes into primate retina

Biraj Mahato, Sanghoon Kim, Houssam Al-Saad, Michael Carlson, Subrata Batabyal, Adnan Dibas, Samarendra K. Mohanty

Author Affiliations +

Proceedings Volume PC11947, Optogenetics and Optical Manipulation 2022; PC1194709 (2022) https://doi.org/10.1117/12.2615466
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Degeneration of photoreceptors, retinal cells including retinal pigment epithelium, and retinal ganglion cells characterizes visual disorders involving retinal dysfunction. The efficient and targeted gene delivery into retinal cells is critical for treatment of a variety of visual disorders. Here, we report use of an OCT guided, spatially targeted, near-infrared laser microirradiation platform to successfully deliver genes encoding ambient-light activatable multi-characteristic opsin (MCO) to retinal cells in non-human primates. The efficacy of gene delivery was evaluated by fundus imaging of the reporter (mCherry) fluorescence, and immunohistochemistry. Functional improvement was measured by red-ERG which was attributed to MCO’s broadband activation spectrum.

Conference Presentation

Citation Download Citation

Biraj Mahato, Sanghoon Kim, Houssam Al-Saad, Michael Carlson, Subrata Batabyal, Adnan Dibas, and Samarendra K. Mohanty "Spatially targeted non-viral optical delivery of opsin-encoding genes into primate retina", Proc. SPIE PC11947, Optogenetics and Optical Manipulation 2022, PC1194709 (7 March 2022); https://doi.org/10.1117/12.2615466

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Retina

Continuous wave operation

Near infrared

Optical coherence tomography

Visualization

Molecules

Target designation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years