3D Hyperspectral light-sheet imaging of pancreatic islets

David W. Piston; Xue Wen Ng; Jiawei Lu; Tomasz Tkaczyk

doi:10.1117/12.2609202

7 March 2022 3D Hyperspectral light-sheet imaging of pancreatic islets

David W. Piston, Xue Wen Ng, Jiawei Lu, Tomasz Tkaczyk

Proceedings Volume PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII; PC1196503 (2022) https://doi.org/10.1117/12.2609202
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

The molecular mechanisms that define cellular functions can be elucidated by live-cell hyperspectral fluorescence microscopy, which permits simultaneous measurements of multiple dynamic processes with excellent signal-to-noise ratios. We have developed a novel five-dimensional (x,y,z,t,λ) imaging system by combining dual-view Selective-Plane Illumination Microscopy (diSPIM) for isotropic diffraction-limited imaging and image mapping spectroscopy (IMS) for single snapshot hyperspectral imaging of multiple fluorophores. We will demonstrate the properties of this instrument and show its application to imaging islet of Langerhans, a micro-organ that contributes to blood glucose homeostasis through the regulated secretion of glucagon (from α-cells) and insulin (from β-cells).

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Citation Download Citation

David W. Piston, Xue Wen Ng, Jiawei Lu, and Tomasz Tkaczyk "3D Hyperspectral light-sheet imaging of pancreatic islets", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC1196503 (7 March 2022); https://doi.org/10.1117/12.2609202

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KEYWORDS

Microscopy

Tissues

Hyperspectral imaging

Luminescence

Signal processing

Optical fibers

Signal to noise ratio

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