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2 March 2022 One-shot highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing
Lixue Shi, Wei Min
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Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC1197305 (2022) https://doi.org/10.1117/12.2607432
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Mapping the localization of multiple proteins in their native 3D context would be useful for biomedicine. We harness the narrow spectrum of Raman spectroscopy and introduce Raman Dye Imaging and Tissue Clearing (RADIANT), an optical method for imaging multiple targets in thick samples in one shot. We expanded the range of bioorthogonal Raman dyes and developed a tissue clearing strategy for them. RADIANT allowed us to image >10 targets in millimeter thick brain slices, extending the imaging depth 10-100 fold compared to prior methods. RADIANT will facilitate the exploration of the intricate 3D protein interactions in complex systems.
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Lixue Shi and Wei Min "One-shot highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC1197305 (2 March 2022); https://doi.org/10.1117/12.2607432
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KEYWORDS
Tissue clearing
Multiplexing
Raman spectroscopy
3D metrology
Proteins
Tissues
Brain mapping
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