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Vibrational imaging based on mid-infrared photothermal (MIP) microscopy with the sub-micrometer spatial resolution is an indispensable analytical tool for a wide range of fields, such as polymer and medical science. In this talk, we will report multimodal imaging of polymer blends and biomolecules combining a MIP imaging with other optical microscopic techniques such as Raman or fluorescence imaging. Since Raman scattering exhibits complementary information of molecular vibrations and fluorescence imaging helps to understand biological dynamics by visualizing the distribution of biomarkers, our demonstration of multimodal imaging offers accurate and detailed characterization of molecular structures and functions of various samples.
Ryo Kato,Taka-aki Yano, andTakuo Tanaka
"Multimodal vibrational imaging based on mid-infrared photothermal microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730I (2 March 2022); https://doi.org/10.1117/12.2607443
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Ryo Kato, Taka-aki Yano, Takuo Tanaka, "Multimodal vibrational imaging based on mid-infrared photothermal microscopy," Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730I (2 March 2022); https://doi.org/10.1117/12.2607443