1Institut de la Vision (France) 2Ctr. Hospitalier National d'Opthalmologie des Quinze-Vingts (France) 3Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France) 4Institut Langevin (France)
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We present the first dynamic full field optical coherence tomography (D-FFOCT) independent module which can be easily integrated into any commercial microscope. Combining the whole setup with a commercial incubator,, we demonstrate three-dimensional live imaging experiments over time periods of minutes to hours to days keeping the sample in healthy conditions. We show timelapse high resolution live images of retinal explants and organoids in disease modeling applications.
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Salvatore Azzollini, Tual Monfort, Olivier Thouvenin, Kate Grieve, "A dynamic full field optical coherence tomography module for in vitro retinal and corneal imaging," Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123670C (9 March 2023); https://doi.org/10.1117/12.2651674