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9 March 2023 Gigantic acousto-optic modulation in multiphoton fluorescence imaging
Justin Little, Behnam Tayebi, Ezra Guralnik, Michal Balberg, Shy Shoham
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Proceedings Volume PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023; PC1237914 (2023) https://doi.org/10.1117/12.2650790
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Demonstrations of acousto-optic effects in fluorescent samples are widespread but their utility is generally limited due to the small amplitude modulations achievable and concurrently, long acquisition times. Here, we report the observation of a novel, gigantic acousto-optic modulation of two-photon excited fluorescence. The effect’s magnitude when applying low intensity ultrasound allows high SNR modulation of fluorescence in standard multiphoton systems without sophisticated detection schemes and without the need for extensive temporal averaging or signal processing. We observe this effect in the brain of awake behaving mice, demonstrating its utility for future advanced AO applications in vivo.
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Justin Little, Behnam Tayebi, Ezra Guralnik, Michal Balberg, and Shy Shoham "Gigantic acousto-optic modulation in multiphoton fluorescence imaging", Proc. SPIE PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023, PC1237914 (9 March 2023); https://doi.org/10.1117/12.2650790
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KEYWORDS
Modulation
Acousto-optics
Multiphoton fluorescence microscopy
Light scattering
Luminescence
Scattering
Tissues
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