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16 March 2023 Large-scale quantitative phase imaging-based single-cell morphological profiling of chemical and genetic perturbations (Conference Presentation)
Dickson Siu, Victor M. L. Wong, Sum Lau, Bei Wang, Alan S. L. Wong, Kenneth K. Y. Wong, Kevin K. Tsia
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Proceedings Volume PC12389, Quantitative Phase Imaging IX; PC123890V (2023) https://doi.org/10.1117/12.2655441
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We developed a large-scale single-cell intrinsic morphological profiling strategy using ultrahigh-throughput quantitative phase imaging (QPI) combined with a novel spinning on-the-fly cell-based assay platform. This integrated system demonstrates the unprecedented functional assay capability of QPI in not only scaling up the assay throughput, but also empowering new cytometric power to perform multiplexed live-cell drug screening (96 conditions in a single run) and genetic perturbation assay (by CRISPR). This platform thus allows generation of large cellular QPI datasets (4.85 TByte in this study) that could spearhead cost-effective label-free solutions for identifying disease-or gene-related cellular morphological phenotypes in therapeutics screening.
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Dickson Siu, Victor M. L. Wong, Sum Lau, Bei Wang, Alan S. L. Wong, Kenneth K. Y. Wong, and Kevin K. Tsia "Large-scale quantitative phase imaging-based single-cell morphological profiling of chemical and genetic perturbations (Conference Presentation)", Proc. SPIE PC12389, Quantitative Phase Imaging IX, PC123890V (16 March 2023); https://doi.org/10.1117/12.2655441
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KEYWORDS
Profiling
Genetics
Luminescence
Receptors
Absorption
Drug discovery
Lung cancer
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