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16 March 2023 Autofluorescence imaging screens of hydrogels for stem cell-derived cardiomyocyte differentiation and maturation (Conference Presentation)
Danielle E. Desa, Margot Amitrano, William L. Murphy, Melissa C. Skala
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Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC1239104 (2023) https://doi.org/10.1117/12.2648379
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Induced pluripotent stem cells (iPSC) can generate patient-specific disease models and drug screening platforms. Heterogeneity in commercial culture substrates causes batch variation in manufactured iPSC cardiomyocyte differentiation and maturation levels. We performed optical metabolic imaging (OMI) of iPSC-cardiomyocytes on polyethylene glycol hydrogels of varying stiffnesses and adhesion ligands to assess the metabolic co-factors NAD(P)H and FAD during differentiation and maturation. Optical redox ratio at 6 days post-differentiation identified hydrogel features most favorable for subsequent cardiomyocyte maturation. These findings indicate that OMI enables rapid, non-invasive screens of favorable culture conditions during early differentiation and may translate to the biomanufacturing industry.
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Danielle E. Desa, Margot Amitrano, William L. Murphy, and Melissa C. Skala "Autofluorescence imaging screens of hydrogels for stem cell-derived cardiomyocyte differentiation and maturation (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC1239104 (16 March 2023); https://doi.org/10.1117/12.2648379
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KEYWORDS
Auto-fluorescence imaging
Manufacturing
Curium
Optical testing
Stem cells
Luminescence
Optical imaging
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