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15 March 2023 Random illumination wide field coherent anti-Stokes Raman scattering microscopy (RIM-CARS) (Conference Presentation)
Hervé Rigneault, Eric Fantuzzi, Sandro Heuke, Randy A. Bartels, Anne Sentenac, Dominykas Gudavičius
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920H (2023) https://doi.org/10.1117/12.2648081
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We revisit wide field CARS imaging using speckle field illuminations. In the proposed scheme we break the CARS coherence using fast varying pump speckle illuminations while keeping static the Stokes speckle. Acquiring a large number of Stokes images enables dynamic speckle illumination (DSI) and random illumination microscopy (RIM). We show that the first one enables quasi-confocal axial sectioning (1/z) while the second one, through post-processing, can retrieve a superior image contrast, noise level and spatial resolution as an important step towards robust nonlinear super-resolution CARS microscopy.
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Hervé Rigneault, Eric Fantuzzi, Sandro Heuke, Randy A. Bartels, Anne Sentenac, and Dominykas Gudavičius "Random illumination wide field coherent anti-Stokes Raman scattering microscopy (RIM-CARS) (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920H (15 March 2023); https://doi.org/10.1117/12.2648081
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KEYWORDS
Microscopy
CARS tomography
Speckle
Coherence imaging
Laser development
Lenses
Objectives
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