Raman projection tomography for label-free 3D molecular imaging of live tissue engineered constructs (Conference Presentation)

Elzbieta Stepula; Anders R. Walther; Dev R. Mehrotra; Magnus Jensen; Michael B. Albro; Martin A. B. Hedegaard; Mads S. Bergholt

doi:10.1117/12.2649318

15 March 2023 Raman projection tomography for label-free 3D molecular imaging of live tissue engineered constructs (Conference Presentation)

Elzbieta Stepula, Anders R. Walther, Dev R. Mehrotra, Magnus Jensen, Michael B. Albro, Martin A. B. Hedegaard, Mads S. Bergholt

Author Affiliations +

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239216 (2023) https://doi.org/10.1117/12.2649318
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Tissue engineering (TE) constructs are an attractive alternative therapy for patients with e.g., degenerated cartilage. The positive patient outcome relies on the quality of the TE constructs as they should mimic the native tissue in its molecular, chemical, and physical properties. Most quality assessment techniques of TE constructs are destructive to the sample. Therefore, there is an urgent need for a novel non-destructive method to control the in vitro cultivating process. Here, we present Raman Projection Tomography (RPT) which enables live label-free 3D molecular imaging. RPT was employed for the non-destructive quality control of cartilage TE constructs.

Conference Presentation

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Elzbieta Stepula, Anders R. Walther, Dev R. Mehrotra, Magnus Jensen, Michael B. Albro, Martin A. B. Hedegaard, and Mads S. Bergholt "Raman projection tomography for label-free 3D molecular imaging of live tissue engineered constructs (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239216 (15 March 2023); https://doi.org/10.1117/12.2649318

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KEYWORDS

Tissues

Raman spectroscopy

Tomography

Molecular imaging

3D image processing

Raman scattering

Scattering

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