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15 March 2023 Low frequency coherent Raman imaging with high optical scattering robustness (Conference Presentation)
David R. Smith, Siddarth Shivkumar, Jeff Field, Jesse Wilson, Hervé Rigneault, Randy Bartels
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239218 (2023) https://doi.org/10.1117/12.2650868
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Raman microscopy is a valuable approach to label-free chemical imaging. Recent advances have enabled the development of rapid methods for hyperspectral microscopy using impulsive stimulated Raman scattering (ISRS) that focus on low-frequency Raman modes of materials. The persistent challenge with Raman spectroscopic methods is low signal due to the relatively weak Raman scattering. ISRS microscopy allows for high quality hyperspectral imaging of low and fingerprint Raman vibrational frequencies but is normally subject to poor performance in the presence of optical scattering. We will present methods that solve this issue and allow for high-quality imaging when strong optical scattering is present.
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David R. Smith, Siddarth Shivkumar, Jeff Field, Jesse Wilson, Hervé Rigneault, and Randy Bartels "Low frequency coherent Raman imaging with high optical scattering robustness (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239218 (15 March 2023); https://doi.org/10.1117/12.2650868
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KEYWORDS
Raman scattering
Coherence imaging
Scattering
Intelligence systems
Raman spectroscopy
Optical imaging
Imaging spectroscopy
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