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17 March 2023 Evaluating the catalytic efficiency of the membrane-type 1 matrix metalloproteinase (MMP-14) using AuNP-peptide assemblies (Conference Presentation)
Hedi Mattoussi, Narjes Dridi, Valle Palomo, Philip E. Dawson, Qing-Xiang Sang, Zhicheng Jin
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Proceedings Volume PC12395, Colloidal Nanoparticles for Biomedical Applications XVIII; PC123950F (2023) https://doi.org/10.1117/12.2650361
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We report on the use of lipoic acid-appended polyethylene glycol (LA-PEG)-stabilized AuNPs, covalently coupled to dye-labelled peptides, as sensitive optically-addressable sensors for determining the catalytic efficiency of the human matrix metalloproteinase-14 (MMP-14), a cancer biomarker. We exploit real-time dye PL recovery triggered by MMP-14 hydrolysis of the AuNP-tethered peptide-dye to extract quantitative analysis of the proteolysis kinetics. Sub-nanomolar limit of detections for MMP-14 has been achieved using our hybrid bioconjugates.
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Hedi Mattoussi, Narjes Dridi, Valle Palomo, Philip E. Dawson, Qing-Xiang Sang, and Zhicheng Jin "Evaluating the catalytic efficiency of the membrane-type 1 matrix metalloproteinase (MMP-14) using AuNP-peptide assemblies (Conference Presentation)", Proc. SPIE PC12395, Colloidal Nanoparticles for Biomedical Applications XVIII, PC123950F (17 March 2023); https://doi.org/10.1117/12.2650361
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KEYWORDS
Nanolithography
Biosensors
Cancer
Luminescence
Nanoparticles
Nanorods
Nanostructures
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