Plasmonic label-free imaging of electrical activity in pancreatic cell (Conference Presentation)

Sidahmed Abayzeed; Paul A. Smith; Finlay Nelson; Oscar G. Barajas-González; Kerry Setchfield; Michael G. Somekh

doi:10.1117/12.2651284

16 March 2023 Plasmonic label-free imaging of electrical activity in pancreatic cell (Conference Presentation)

Sidahmed Abayzeed, Paul A. Smith, Finlay Nelson, Oscar G. Barajas-González, Kerry Setchfield, Michael G. Somekh

Author Affiliations +

Proceedings Volume PC12396, Plasmonics in Biology and Medicine XX; PC123960C (2023) https://doi.org/10.1117/12.2651284
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

This paper presents a widefield label-free plasmonic method for imaging electrical activity of pancreatic beta cells. MIN6 insulinoma cells were grown on gold surfaces modified with poly-L-lysine to promote adhesion. The results presented show synchronised electrical signals upon extracellular exposure to 10mM glucose solution. Spatiotemporal patterns of electrical activity are recorded with a sampling frequency of 100Hz and the microscopic technique offers a signal-to-noise ratio that enables measurements from an area as small as 1µm2.

Conference Presentation

Citation Download Citation

Sidahmed Abayzeed, Paul A. Smith, Finlay Nelson, Oscar G. Barajas-González, Kerry Setchfield, and Michael G. Somekh "Plasmonic label-free imaging of electrical activity in pancreatic cell (Conference Presentation)", Proc. SPIE PC12396, Plasmonics in Biology and Medicine XX, PC123960C (16 March 2023); https://doi.org/10.1117/12.2651284

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