Thrombus formation in a blood vessel-on-chip with visible-light optical coherence tomography

Carlos Cuartas-Vélez; Heleen H. T. Middelkamp; Andries van der Meer; Albert van den Berg; Nienke Bosschaart

doi:10.1117/12.3001494

13 March 2024 Thrombus formation in a blood vessel-on-chip with visible-light optical coherence tomography

Carlos Cuartas-Vélez, Heleen H. T. Middelkamp, Andries van der Meer, Albert van den Berg, Nienke Bosschaart

Proceedings Volume PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; PC1283705 (2024) https://doi.org/10.1117/12.3001494
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Thrombus formation is a physiological response to damage in a blood vessel. Monitoring thrombus formation is challenging, due to the limited imaging options available to analyze flowing blood. In this work, we use a visible-light optical coherence tomography (vis-OCT) system to monitor the dynamic process of the formation of thrombi in a microfluidic blood vessel-on-chip (VoC) device. Inside the VoC, thrombi form in a monolayered channel of endothelial cells perfused by human whole blood. We show that the correlation of the vis-OCT signal can be utilized as a marker for thrombus formation and can track and quantify its growth over time. We validate our results with fluorescence microscopic imaging of fibrin and platelets.

Conference Presentation

Citation Download Citation

Carlos Cuartas-Vélez, Heleen H. T. Middelkamp, Andries van der Meer, Albert van den Berg, and Nienke Bosschaart "Thrombus formation in a blood vessel-on-chip with visible-light optical coherence tomography", Proc. SPIE PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, PC1283705 (13 March 2024); https://doi.org/10.1117/12.3001494

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Blood

Optical coherence tomography

Biological imaging

Blood circulation

Microscopy

Optical sensing

Perfusion imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years