Transcranial photoacoustic imaging with ICG J-aggregates for imaging blood-brain barrier leak following LPS-induced neuroinflammation

Filip J. Bodera; Shrishti Singh; Remi Veneziano; Parag V. Chitnis; Mark J. McVey; Michael C. Kolios

doi:10.1117/12.3002919

13 March 2024 Transcranial photoacoustic imaging with ICG J-aggregates for imaging blood-brain barrier leak following LPS-induced neuroinflammation

Filip J. Bodera, Shrishti Singh, Remi Veneziano, Parag V. Chitnis, Mark J. McVey, Michael C. Kolios

Author Affiliations +

Proceedings Volume PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024; PC128421X (2024) https://doi.org/10.1117/12.3002919
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Transcranial photoacoustic imaging shows promise for non-invasive evaluation of brain injury and blood-brain barrier disruption (BBB-D). Our study used neonatal rats with immature cerebral blood vessels, making them more susceptible to brain injury. Neuroinflammation was induced using lipopolysaccharide (LPS), leading to BBB-D. We employed a small-animal photoacoustic imaging system that integrated a wavelength-tunable laser (680-970 nm) and a high-frequency ultrasound transducer to obtain transcranial ultrasound and photoacoustic (PA) images. BBB-D was visualized by the migration and accumulation of indocyanine green (ICG) J-aggregate nanoprobes in the brain, resulting in enhanced PA signal. Following LPS injection, a two-fold increase in PA signal intensity was observed at 2 hours, peaking at a four-fold increase at 4 hours. The enhanced PA signal persisted up to 24 hours and remained within 30% of the baseline at 48 hours. These findings have significant implications for early detection of BBB-D using transcranial photoacoustic imaging, made possible by the use of neonatal rats with thin skulls and photoacoustic contrast agents with distinct spectral signatures in vivo.

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Filip J. Bodera, Shrishti Singh, Remi Veneziano, Parag V. Chitnis, Mark J. McVey, and Michael C. Kolios "Transcranial photoacoustic imaging with ICG J-aggregates for imaging blood-brain barrier leak following LPS-induced neuroinflammation", Proc. SPIE PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024, PC128421X (13 March 2024); https://doi.org/10.1117/12.3002919

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