Profiling the effects and mechanisms of immunopotentiator GC on myeloid cells within the tumor microenvironment using single-cell RNA sequencing (scRNAseq)

Kaili Liu; Jacob Adams; Robert A. Miller; Yuanhong Sun; Wei R. Chen

doi:10.1117/12.3004196

13 March 2024 Profiling the effects and mechanisms of immunopotentiator GC on myeloid cells within the tumor microenvironment using single-cell RNA sequencing (scRNAseq)

Kaili Liu, Jacob Adams, Robert A. Miller, Yuanhong Sun, Wei R. Chen

Author Affiliations +

Proceedings Volume PC12843, Biophotonics and Immune Responses XIX; PC1284305 (2024) https://doi.org/10.1117/12.3004196
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Investigating myeloid cells' role during cancer therapy is critical to developing novel strategies, as they shape the tumor microenvironment and modulate anti-tumor immune responses. Using single-cell RNA sequencing (scRNAseq), we examined the impact of the immunostimulant N-dihydrogalactochitosan (GC) on myeloid cells within MMTV-PyMT tumors. We discovered unique myeloid cell clusters with varied responses to GC, showing increased proportions of certain cell types, such as G-MDSC, monocytes, and DCs. Importantly, we observed significant upregulation of STING signaling-associated genes, indicative of conventional STING signaling and canonical NFkB signaling activation. Furthermore, our analysis showed an upregulation of proinflammatory cytokines in cDCs, and a significant reduction in M2-like macrophages post-GC treatment. This supports GC's potent immunostimulatory properties, activating key cells within the tumor microenvironment to enhance antitumor immunity.

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Kaili Liu, Jacob Adams, Robert A. Miller, Yuanhong Sun, and Wei R. Chen "Profiling the effects and mechanisms of immunopotentiator GC on myeloid cells within the tumor microenvironment using single-cell RNA sequencing (scRNAseq)", Proc. SPIE PC12843, Biophotonics and Immune Responses XIX, PC1284305 (13 March 2024); https://doi.org/10.1117/12.3004196

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KEYWORDS

Tumors

Cell phenotyping

Profiling

Modulation

Molecules

Principal component analysis

Ablation

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