3D isotropic light-sheet imaging to reveal the structure of neonatal mouse hearts

Milad Almasian; Xinyuan Zhang; Alireza Saberigarakani; Yichen Ding

doi:10.1117/12.3002774

13 March 2024 3D isotropic light-sheet imaging to reveal the structure of neonatal mouse hearts

Milad Almasian, Xinyuan Zhang, Alireza Saberigarakani, Yichen Ding

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC128480F (2024) https://doi.org/10.1117/12.3002774
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The neonatal mouse heart in contrast to the adults has the ability to regenerate after myocardial infarction. The investigation of cardiac morphogenesis is critical to uncover the underlying mechanism of cardiac regeneration. Hence, we have developed a light-sheet microscope (LSM) along with the tissue-clearing method to investigate the 3-dimensional (3D) architecture of the intact neonatal mouse heart. We improved our imaging system by incorporating an axially swept remote focusing arm with a voice coil actuator for isotropic imaging. Our LSM offers a lateral resolution of 2.08 ± 0.11 μm and an axial resolution of 2.84 ± 0.19 μm. Using this strategy, we can take optical sections throughout the intact heart and enabling us to reveal the cardiac structure of the neonatal mouse.

Conference Presentation

Citation Download Citation

Milad Almasian, Xinyuan Zhang, Alireza Saberigarakani, and Yichen Ding "3D isotropic light-sheet imaging to reveal the structure of neonatal mouse hearts", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC128480F (13 March 2024); https://doi.org/10.1117/12.3002774

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