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13 March 2024 Reflectin proteins as biomolecular reporters for quantitative phase imaging
Nikhil Kaimal, Georgii Bogdanov, Aleeza Farrukh, Alon A. Gorodetsky
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Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520R (2024) https://doi.org/10.1117/12.3001899
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
During the last several decades, cephalopods have emerged as a powerful source of inspiration for the engineering of dynamic optical systems. Previously, we drew inspiration from the tunable optical functionality of cephalopod skin cells for the engineering of human cells to produce high refractive index subcellular architectures consisting of the cephalopod protein reflectin. Additionally, we investigated the self-assembly, optical properties, and stimuli responsiveness of various reflectin isoforms in-vitro, utilizing three-dimensional holotomographic microscopy. More recently, we have obtained improved control over the production of reflectin in human cells by genetically engineering cell lines to stably express reflectins. Furthermore, we have systematically interrogated the optical properties of reflectin-expressing cells with three-dimensional holotomographic microscopy and harnessed their high-contrast reflectin subcellular structures to track cell migration (2,3). The combined findings have indicated that reflectins could find applications as genetically encoded high-contrast biomolecular reporters for the study of cellular-level processes.
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Nikhil Kaimal, Georgii Bogdanov, Aleeza Farrukh, and Alon A. Gorodetsky "Reflectin proteins as biomolecular reporters for quantitative phase imaging", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520R (13 March 2024); https://doi.org/10.1117/12.3001899
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KEYWORDS
Reflection
Biomolecules
Proteins
Phase imaging
Engineering
Microscopy
Optical properties
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