Label-free imaging and virtual staining of human prostate paraffin sections for abnormality and cancer diagnosis

Mingxuan SI; Weixing Dai; Yan Zhang; Hei Man Wong; Tsz Wai Wong

doi:10.1117/12.2691299

13 March 2024 Label-free imaging and virtual staining of human prostate paraffin sections for abnormality and cancer diagnosis

Mingxuan SI, Weixing Dai, Yan Zhang, Hei Man Wong, Tsz Wai Wong

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285401 (2024) https://doi.org/10.1117/12.2691299
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

In this work, we propose a deep-UV LED-based imaging modality for generating AF images on human prostate paraffin sections. The achieved lateral resolution is around 1 µm. Moreover, a virtual staining framework is used to style transfer the AF images into clinical standard H&E images with an MS-SSIM > 0.65 between the virtually stained images and their corresponding ground truth images. The high similarity between the two domains is recognized by medical professionals, illustrating the high accuracy of the virtual staining algorithm and the clinical importance of this work for the post-operative histopathology workflow.

Conference Presentation

Citation Download Citation

Mingxuan SI, Weixing Dai, Yan Zhang, Hei Man Wong, and Tsz Wai Wong "Label-free imaging and virtual staining of human prostate paraffin sections for abnormality and cancer diagnosis", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285401 (13 March 2024); https://doi.org/10.1117/12.2691299

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