Label-free deep imaging inside scattering media through virtual spatiotemporal wavefront shaping

Yiwen Zhang; Minh Dinh; Zeyu Wang; Tianhao Zhang; Chia Wei Hsu; Tianhang Chen

doi:10.1117/12.2685661

13 March 2024 Label-free deep imaging inside scattering media through virtual spatiotemporal wavefront shaping

Yiwen Zhang, Minh Dinh, Zeyu Wang, Tianhao Zhang, Chia Wei Hsu, Tianhang Chen

Proceedings Volume PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences; PC1285714 (2024) https://doi.org/10.1117/12.2685661
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Wawefront shaping can overcome light scattering to focus deep inside turbid media, but it typically requires feedback from guidestars and is restricted by the isoplanatic patch size and modulation speed. Here, we present scattering matrix tomography (SMT) that uses the measured scattering matrix to perform virtual spatiotemporal wavefront shaping, with a digitally scanned confocal spatiotemporal focus and guidestar-free wavefront optimization for every isoplanatic patch. We experimentally use SMT to achieve diffraction-limited resolution behind one-millimeter-thick ex-vivo mouse brain tissue which reduces the target signal by over ten million-fold. We also realize 3D tomography with ideal transverse and axial resolutions inside a dense colloid, where conventional imaging methods fail due to multiple scattering, across a large depth of field of over 70 times the Rayleigh range.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yiwen Zhang, Minh Dinh, Zeyu Wang, Tianhao Zhang, Chia Wei Hsu, and Tianhang Chen "Label-free deep imaging inside scattering media through virtual spatiotemporal wavefront shaping", Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC1285714 (13 March 2024); https://doi.org/10.1117/12.2685661

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