Circularly polarized SRS imaging of cells and tissues

Zhi Li; Hongje Jang; Yajuan Li; Anthony A. Fung; Yu Ping; Lingyan Shi

doi:10.1117/12.3009032

13 March 2024 Circularly polarized SRS imaging of cells and tissues

Zhi Li, Hongje Jang, Yajuan Li, Anthony A. Fung, Yu Ping, Lingyan Shi

Proceedings Volume PC12901, Complex Light and Optical Forces XVIII; PC129010K (2024) https://doi.org/10.1117/12.3009032
Event: SPIE OPTO, 2024, San Francisco, California, United States

Abstract

The extensively employed technique of Stimulated Raman Scattering (SRS) has found widespread application in the domain of biomedical imaging. Typically, the CH band is harnessed for the identification of biomolecules. Nonetheless, the task of distinguishing between CH2 and CH3 bonds presents an ongoing challenge. In the present investigation, Circularly Polarized Stimulated Raman Scattering (CP-SRS) illumination was employed. This was carried out to explore the impact of polarization on the differentiation of CH vibrational modes. Intriguingly, it was observed that circular polarization notably repressed CH2 bonds in contrast to CH3 bonds. This selective effect facilitated the visualization of pure protein, eschewing the complexities posed by protein-lipid compounds. This unique unmixing approach significantly enhances the chemical specificity of SRS imaging. As a result, it holds remarkable implications for applications such as metabolic imaging and beyond.

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Citation Download Citation

Zhi Li, Hongje Jang, Yajuan Li, Anthony A. Fung, Yu Ping, and Lingyan Shi "Circularly polarized SRS imaging of cells and tissues", Proc. SPIE PC12901, Complex Light and Optical Forces XVIII, PC129010K (13 March 2024); https://doi.org/10.1117/12.3009032

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KEYWORDS

Biological imaging

Tissues

Vibration

Polarization

Biological research

Chemical analysis

Proteins

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