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1 May 2006 Investigation of living pancreas tumor cells by digital holographic microscopy
Björn Kemper, Daniel D. Carl, Jürgen Schnekenburger, Ilona Bredebusch, Marcus Schäfer, Wolfram Domschke, Gert von Bally
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Journal of Biomedical Optics, Vol. 11, Issue 3, 034005 (May 2006). https://doi.org/10.1117/1.2204609
Abstract
Digital holographic microscopy provides new facilities for contactless and marker-free quantitative phase contrast imaging. In this work, a digital holographic microscopy method for the integral refractive index determination of living single cells in cell culture medium is presented. Further, the obtained refractive index information is applied to full field thickness and shape determination of adherent pancreas tumor cells, as well as for analysis of drug-induced dynamic changes of a single cell's cytoskeleton. The results demonstrate that digital holographic microscopy is a quantitative phase contrast technique for living cells under conventional laboratory conditions.
©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Björn Kemper, Daniel D. Carl, Jürgen Schnekenburger, Ilona Bredebusch, Marcus Schäfer, Wolfram Domschke, and Gert von Bally "Investigation of living pancreas tumor cells by digital holographic microscopy," Journal of Biomedical Optics 11(3), 034005 (1 May 2006). https://doi.org/10.1117/1.2204609
Published: 1 May 2006
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Cited by 338 scholarly publications and 9 patents.
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KEYWORDS
Digital holography
Holography
Refractive index
Microscopy
3D image reconstruction
Holograms
Phase contrast
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