CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 15 > Issue 2 > Article
Open Access
1 March 2010 Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity
Christophe Pache, Jonas Kühn, Kriss Westphal, Muhammed Fatih Toy, Jérôme Parent, Oralea Buchi, Alfredo Franco-Obregon, Christian D. Depeursinge, Marcel Egli
Author Affiliations +
Journal of Biomedical Optics, Vol. 15, Issue 2, 026021 (March 2010). https://doi.org/10.1117/1.3377960
Abstract
Previous investigations on mammalian cells have shown that microgravity, either that experienced in space, or simulated on earth, causes severe cellular modifications that compromise tissue determination and function. The aim of this study is to investigate, in real time, the morphological changes undergone by cells experiencing simulated microgravity by using digital holographic microscopy (DHM). DHM analysis of living mouse myoblasts (C2C12) is undertaken under simulated microgravity with a random positioning machine. The DHM analysis reveals cytoskeletal alterations similar to those previously reported with conventional methods, and in agreement with conventional brightfield fluorescence microscopy a posteriori investigation. Indeed, DHM is shown to be able to noninvasively and quantitatively detect changes in actin reticular formation, as well as actin distribution, in living unstained samples. Such results were previously only obtainable with the use of labeled probes in conjunction with conventional fluorescence microscopy, with all the classically described limitations in terms of bias, bleaching, and temporal resolution.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation Download Citation
Christophe Pache, Jonas Kühn, Kriss Westphal, Muhammed Fatih Toy, Jérôme Parent, Oralea Buchi, Alfredo Franco-Obregon, Christian D. Depeursinge, and Marcel Egli "Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity," Journal of Biomedical Optics 15(2), 026021 (1 March 2010). https://doi.org/10.1117/1.3377960
Published: 1 March 2010
JOURNAL ARTICLE
 9 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 30 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Microscopy
Digital holography
Cytoskeletons
Luminescence
Computer simulations
Holography
Proteins
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top