Optical spectral imaging of degeneration of articular cartilage

Jussi Kinnunen; Jukka S. Jurvelin; Jaana Mäkitalo; Markku Hauta-Kasari; Pasi Vahimaa; Simo Saarakkala

doi:10.1117/1.3477190

1 July 2010 Optical spectral imaging of degeneration of articular cartilage

Jussi Kinnunen, Jukka S. Jurvelin, Jaana Mäkitalo, Markku Hauta-Kasari, Pasi Vahimaa, Simo Saarakkala

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Journal of Biomedical Optics, Vol. 15, Issue 4, 046024 (July 2010). https://doi.org/10.1117/1.3477190

Abstract

Osteoarthritis (OA) is a common musculoskeletal disorder often diagnosed during arthroscopy. In OA, visual color changes of the articular cartilage surface are typically observed. We demonstrate in vitro the potential of visible light spectral imaging (420 to 720 nm) to quantificate these color changes. Intact bovine articular cartilage samples (n=26) are degraded both enzymatically using the collagenase and mechanically using the emery paper (P60 grit, 269 µm particle size). Spectral images are analyzed by using standard CIELAB color coordinates and the principal component analysis (PCA). After collagenase digestion, changes in the CIELAB coordinates and projection of the spectra to PCA eigenvector are statistically significant (p<0.05). After mechanical degradation, the grinding tracks could not be visualized in the RGB presentation, i.e., in the visual appearance of the sample to the naked eye under the D65 illumination. However, after projecting to the chosen eigenvector, the grinding tracks are revealed. The tracks are also seen by using only one wavelength, i.e., 469 nm, however, the contrast in the projection image is 1.6 to 2.5 times higher. Our results support the idea that the spectral imaging can be used for evaluation of the integrity of the cartilage surface.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Jussi Kinnunen, Jukka S. Jurvelin, Jaana Mäkitalo, Markku Hauta-Kasari, Pasi Vahimaa, and Simo Saarakkala "Optical spectral imaging of degeneration of articular cartilage," Journal of Biomedical Optics 15(4), 046024 (1 July 2010). https://doi.org/10.1117/1.3477190

Published: 1 July 2010

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