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1 August 2011 Transillumination hyperspectral imaging for histopathological examination of excised tissue
Fartash Vasefi, Mohamadreza Najiminaini, Eldon Ng, Astrid Chamson-Reig, Jeffrey J. L. Carson, Bozena Kaminska, Muriel Brackstone
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Journal of Biomedical Optics, Vol. 16, Issue 8, 086014 (August 2011). https://doi.org/10.1117/1.3623410
Abstract
Angular domain spectroscopic imaging (ADSI) is a novel technique for the detection and characterization of optical contrast in turbid media based on spectral characteristics. The imaging system employs a silicon micromachined angular filter array to reject scattered light traversing a specimen and an imaging spectrometer to capture and discriminate the largely remaining quasiballistic light based on spatial position and wavelength. The imaging modality results in hyperspectral shadowgrams containing two-dimensional (2D) spatial maps of spectral information. An ADSI system was constructed and its performance was evaluated in the near-infrared region on tissue-mimicking phantoms. Image-based spectral correlation analysis using transmission spectra and first order derivatives revealed that embedded optical targets could be resolved. The hyperspectral images obtained with ADSI were observed to depend on target concentration, target depth, and scattering level of the background medium. A similar analysis on a muscle and tumor sample dissected from a mouse resulted in spatially dependent optical transmission spectra that were distinct, which suggested that ADSI may find utility in classifying tissues in biomedical applications.
©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Fartash Vasefi, Mohamadreza Najiminaini, Eldon Ng, Astrid Chamson-Reig, Jeffrey J. L. Carson, Bozena Kaminska, and Muriel Brackstone "Transillumination hyperspectral imaging for histopathological examination of excised tissue," Journal of Biomedical Optics 16(8), 086014 (1 August 2011). https://doi.org/10.1117/1.3623410
Published: 1 August 2011
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Cited by 15 scholarly publications and 1 patent.
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KEYWORDS
Tissues
Tissue optics
Tumors
Scattering
Absorption
Hyperspectral imaging
Spectroscopy
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