Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system

Weirong Mo; Daniel J. Rohrbach; Ulas Sunar

doi:10.1117/1.JBO.17.7.071306

16 May 2012 Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system

Weirong Mo, Daniel J. Rohrbach, Ulas Sunar

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Journal of Biomedical Optics, Vol. 17, Issue 7, 071306 (May 2012). https://doi.org/10.1117/1.JBO.17.7.071306

Abstract

We report the tomographic imaging of a photodynamic therapy (PDT) photosensitizer, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) in vivo with time-domain fluorescence diffuse optical tomography (TD-FDOT). Simultaneous reconstruction of fluorescence yield and lifetime of HPPH was performed before and after PDT. The methodology was validated in phantom experiments, and depth-resolved in vivo imaging was achieved through simultaneous three-dimensional (3-D) mappings of fluorescence yield and lifetime contrasts. The tomographic images of a human head-and-neck xenograft in a mouse confirmed the preferential uptake and retention of HPPH by the tumor 24-h post-injection. HPPH-mediated PDT induced significant changes in fluorescence yield and lifetime. This pilot study demonstrates that TD-FDOT may be a good imaging modality for assessing photosensitizer distributions in deep tissue during PDT monitoring.

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Weirong Mo, Daniel J. Rohrbach, and Ulas Sunar "Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system," Journal of Biomedical Optics 17(7), 071306 (16 May 2012). https://doi.org/10.1117/1.JBO.17.7.071306

Published: 16 May 2012

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