Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors

Nora Bloise; Gabriele Ceccarelli; Paolo Minzioni; Marco Vercellino; Laura Benedetti; Maria Gabriella C. De Angelis; Marcello Imbriani; Livia Visai

doi:10.1117/1.JBO.18.12.128006

23 December 2013 Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors

Nora Bloise, Gabriele Ceccarelli, Paolo Minzioni, Marco Vercellino, Laura Benedetti, Maria Gabriella C. De Angelis, Marcello Imbriani, Livia Visai

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Journal of Biomedical Optics, Vol. 18, Issue 12, 128006 (December 2013). https://doi.org/10.1117/1.JBO.18.12.128006

Abstract

Several studies have shown that low-level laser irradiation (LLLI) has beneficial effects on bone regeneration. The objective of this study was to examine the in vitro effects of LLLI on proliferation and differentiation of a human osteoblast-like cell line (Saos-2 cell line). Cultured cells were exposed to different doses of LLLI with a semiconductor diode laser (659 nm; 10 mW power output). The effects of laser on proliferation were assessed daily up to seven days of culture in cells irradiated once or for three consecutive days with laser doses of 1 or 3 J/cm ² . The obtained results showed that laser stimulation enhances the proliferation potential of Saos-2 cells without changing their telomerase pattern or morphological characteristics. The effects on cell differentiation were assessed after three consecutive laser irradiation treatments in the presence or absence of osteo-inductive factors on day 14. Enhanced secretion of proteins specific for differentiation toward bone as well as calcium deposition and alkaline phosphatase activity were observed in irradiated cells cultured in a medium not supplemented with osteogenic factors. Taken together these findings indicate that laser treatment enhances the in vitro proliferation of Saos-2 cells, and also influences their osteogenic maturation, which suggest it is a helpful application for bone tissue regeneration.

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Nora Bloise, Gabriele Ceccarelli, Paolo Minzioni, Marco Vercellino, Laura Benedetti, Maria Gabriella C. De Angelis, Marcello Imbriani, and Livia Visai "Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors," Journal of Biomedical Optics 18(12), 128006 (23 December 2013). https://doi.org/10.1117/1.JBO.18.12.128006

Published: 23 December 2013

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