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9 May 2013 Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel
Hamideh Salehi, Elodie Middendorp, Ivan Panayotov, Pierre-Yves Collard Dutilleul, Attila-Gergely Vegh, Sathish Kumar Ramakrishnan, Csilla Gergely, Frédéric J. G. Cuisinier
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Journal of Biomedical Optics, Vol. 18, Issue 5, 056010 (May 2013). https://doi.org/10.1117/1.JBO.18.5.056010
Abstract
Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cytoplasm, indicating cell apoptosis via a caspase independent pathway.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2013/$25.00 © 2013 SPIE
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Hamideh Salehi, Elodie Middendorp, Ivan Panayotov, Pierre-Yves Collard Dutilleul, Attila-Gergely Vegh, Sathish Kumar Ramakrishnan, Csilla Gergely, and Frédéric J. G. Cuisinier "Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel," Journal of Biomedical Optics 18(5), 056010 (9 May 2013). https://doi.org/10.1117/1.JBO.18.5.056010
Published: 9 May 2013
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Cited by 27 scholarly publications.
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KEYWORDS
Raman spectroscopy
Cell death
Confocal microscopy
Data analysis
Microscopy
Data acquisition
Biological research
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