Tomographic phase microscopy of living three-dimensional cell cultures

Arkadiusz Kuś; Michał Dudek; Björn Kemper; Małgorzata Kujawińska; Angelika Vollmer

doi:10.1117/1.JBO.19.4.046009

10 April 2014 Tomographic phase microscopy of living three-dimensional cell cultures

Arkadiusz Kuś, Michał Dudek, Björn Kemper, Małgorzata Kujawińska, Angelika Vollmer

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Journal of Biomedical Optics, Vol. 19, Issue 4, 046009 (April 2014). https://doi.org/10.1117/1.JBO.19.4.046009

Abstract

A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical fiber as the sample cuvette, the observation of living cells in different 3-D matrices is enabled. The fiber delivers a stable and accurate rotation of a cell or cell cluster, providing quantitative phase data for tomographic reconstruction of the 3-D refractive index distribution with an isotropic spatial resolution. We demonstrate that it is possible to clearly distinguish and quantitatively analyze several cells grouped in a “3-D cluster” as well as subcellular organelles like the nucleoli and local internal refractive index changes.

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Arkadiusz Kuś, Michał Dudek, Björn Kemper, Małgorzata Kujawińska, and Angelika Vollmer "Tomographic phase microscopy of living three-dimensional cell cultures," Journal of Biomedical Optics 19(4), 046009 (10 April 2014). https://doi.org/10.1117/1.JBO.19.4.046009

Published: 10 April 2014

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KEYWORDS

Refractive index

Tomography

Digital holography

Microscopy

Capillaries

Collagen

Microscopes

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