Noninvasive photoacoustic microscopy of methemoglobin in vivo

Min Tang; Yong Zhou; Ruiying Zhang; Lihong V. Wang

doi:10.1117/1.JBO.20.3.036007

11 March 2015 Noninvasive photoacoustic microscopy of methemoglobin in vivo

Min Tang, Yong Zhou, Ruiying Zhang, Lihong V. Wang

Author Affiliations +

Funded by: National Institutes of Health (NIH), National Institutes of Health, NIH, National Institute of Health, National Academies Keck Futures Initiative grant IS 13, and National Institutes of Health, National Institute of Health (NIH), US National Institutes of Health (NIH), National Institute of Health or Wellcome Trust, NIH Director’s Pioneer Award, (NIH Director’s Pioneer Award), NIH Director’s Transformative Research Award, (NIH Director’s Transformative Research Award), BRAIN Initiative, National Institutes of Health Grant, National Institutes of Health grants

Journal of Biomedical Optics, Vol. 20, Issue 3, 036007 (March 2015). https://doi.org/10.1117/1.JBO.20.3.036007

Abstract

Due to the various causes of methemoglobinemia and its potential to be confused with other diseases, in vivo measurements of methemoglobin have significant applications in the clinic. Using photoacoustic microscopy (PAM), we quantified the average and the distributed percentage of methemoglobin both in vitro and in vivo. Based on the absorption spectra of methemoglobin, oxyhemoglobin, and deoxyhemoglobin, three wavelengths were chosen to differentiate methemoglobin from the others. The methemoglobin concentrations calculated from the photoacoustic signals agreed well with the preset concentrations. Then we imaged the methemoglobin percentage in microtubes that mimicked blood vessels. Average percentages calculated for five samples with different methemoglobin concentrations also agreed well with the preset values. Finally, we demonstrated the ability of PAM to detect methemoglobin in vivo in a mouse ear. Our results show that PAM can quantitatively image methemoglobin distribution in vivo.

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Min Tang, Yong Zhou, Ruiying Zhang, and Lihong V. Wang "Noninvasive photoacoustic microscopy of methemoglobin in vivo," Journal of Biomedical Optics 20(3), 036007 (11 March 2015). https://doi.org/10.1117/1.JBO.20.3.036007

Published: 11 March 2015

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Cited by 17 scholarly publications and 2 patents.

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KEYWORDS

In vivo imaging

Blood

Photoacoustic microscopy

Photoacoustic spectroscopy

Sodium

Absorption

Blood vessels

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