Optical properties of mice skull bone in the 455- to 705-nm range

Haleh Soleimanzad; Hirac Gurden; Frédéric Pain

doi:10.1117/1.JBO.22.1.010503

31 January 2017 Optical properties of mice skull bone in the 455- to 705-nm range

Haleh Soleimanzad, Hirac Gurden, Frédéric Pain

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Journal of Biomedical Optics, Vol. 22, Issue 1, 010503 (January 2017). https://doi.org/10.1117/1.JBO.22.1.010503

Abstract

Rodent brain is studied to understand the basics of brain function. The activity of cell populations and networks is commonly recorded in vivo with wide-field optical imaging techniques such as intrinsic optical imaging, fluorescence imaging, or laser speckle imaging. These techniques were recently adapted to unrestrained mice carrying transcranial windows. Furthermore, optogenetics studies would benefit from optical stimulation through the skull without implanting an optical fiber, especially for longitudinal studies. In this context, the knowledge of bone optical properties is requested to improve the quantitation of the depth and volume of imaged or stimulated tissues. Here, we provide experimental measurements of absorption and reduced scattering coefficients of freshly excised mice skull for wavelengths between 455 and 705 nm. Absorption coefficients from 6 to 8 months mice skull samples range between 1.67±0.28 mm−1 at 455 nm and 0.47±0.07 mm−1 at 705 nm, whereas reduced scattering coefficients were in the range of 2.79±0.26 mm−1 at 455 nm up to 2.29±0.12 mm−1 at 705 nm. In comparison, measurements carried out on 4 to 5 weeks mice showed similar spectral profiles but smaller absorption and reduced scattering coefficients by a factor of about 2 and 1.5, respectively.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Haleh Soleimanzad, Hirac Gurden, and Frédéric Pain "Optical properties of mice skull bone in the 455- to 705-nm range," Journal of Biomedical Optics 22(1), 010503 (31 January 2017). https://doi.org/10.1117/1.JBO.22.1.010503

Received: 3 August 2016; Accepted: 4 January 2017; Published: 31 January 2017

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