Quantitative real-time optical imaging of the tissue metabolic rate of oxygen consumption

Michael T. Ghijsen; Griffin R. Lentsch; Sylvain Gioux; Matthew Brenner M.D.; Anthony J. Durkin; Bernard Choi; Bruce J. Tromberg

doi:10.1117/1.JBO.23.3.036013

24 March 2018 Quantitative real-time optical imaging of the tissue metabolic rate of oxygen consumption

Michael T. Ghijsen, Griffin R. Lentsch, Sylvain Gioux, Matthew Brenner M.D., Anthony J. Durkin, Bernard Choi, Bruce J. Tromberg

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Journal of Biomedical Optics, Vol. 23, Issue 3, 036013 (March 2018). https://doi.org/10.1117/1.JBO.23.3.036013

Abstract

The tissue metabolic rate of oxygen consumption (tMRO2) is a clinically relevant marker for a number of pathologies including cancer and arterial occlusive disease. We present and validate a noncontact method for quantitatively mapping tMRO2 over a wide, scalable field of view at 16 frames / s. We achieve this by developing a dual-wavelength, near-infrared coherent spatial frequency-domain imaging (cSFDI) system to calculate tissue optical properties (i.e., absorption, μa, and reduced scattering, μs′, parameters) as well as the speckle flow index (SFI) at every pixel. Images of tissue oxy- and deoxyhemoglobin concentration ( [ HbO2 ] and [HHb]) are calculated from optical properties and combined with SFI to calculate tMRO2. We validate the system using a series of yeast-hemoglobin tissue-simulating phantoms and conduct in vivo tests in humans using arterial occlusions that demonstrate sensitivity to tissue metabolic oxygen debt and its repayment. Finally, we image the impact of cyanide exposure and toxicity reversal in an in vivo rabbit model showing clear instances of mitochondrial uncoupling and significantly diminished tMRO2. We conclude that dual-wavelength cSFDI provides rapid, quantitative, wide-field mapping of tMRO2 that can reveal unique spatial and temporal dynamics relevant to tissue pathology and viability.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Michael T. Ghijsen, Griffin R. Lentsch, Sylvain Gioux, Matthew Brenner M.D., Anthony J. Durkin, Bernard Choi, and Bruce J. Tromberg "Quantitative real-time optical imaging of the tissue metabolic rate of oxygen consumption," Journal of Biomedical Optics 23(3), 036013 (24 March 2018). https://doi.org/10.1117/1.JBO.23.3.036013

Received: 12 November 2017; Accepted: 28 February 2018; Published: 24 March 2018

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