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9 April 2018 Method using in vivo quantitative spectroscopy to guide design and optimization of low-cost, compact clinical imaging devices: emulation and evaluation of multispectral imaging systems
Rolf B. Saager, Melissa L. Baldado, Rebecca A. Rowland, Kristen M. Kelly, Anthony J. Durkin
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Journal of Biomedical Optics, Vol. 23, Issue 4, 046002 (April 2018). https://doi.org/10.1117/1.JBO.23.4.046002
Abstract
With recent proliferation in compact and/or low-cost clinical multispectral imaging approaches and commercially available components, questions remain whether they adequately capture the requisite spectral content of their applications. We present a method to emulate the spectral range and resolution of a variety of multispectral imagers, based on in-vivo data acquired from spatial frequency domain spectroscopy (SFDS). This approach simulates spectral responses over 400 to 1100 nm. Comparing emulated data with full SFDS spectra of in-vivo tissue affords the opportunity to evaluate whether the sparse spectral content of these imagers can (1) account for all sources of optical contrast present (completeness) and (2) robustly separate and quantify sources of optical contrast (crosstalk). We validate the approach over a range of tissue-simulating phantoms, comparing the SFDS-based emulated spectra against measurements from an independently characterized multispectral imager. Emulated results match the imager across all phantoms (<3  %   absorption, <1  %   reduced scattering). In-vivo test cases (burn wounds and photoaging) illustrate how SFDS can be used to evaluate different multispectral imagers. This approach provides an in-vivo measurement method to evaluate the performance of multispectral imagers specific to their targeted clinical applications and can assist in the design and optimization of new spectral imaging devices.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Rolf B. Saager, Melissa L. Baldado, Rebecca A. Rowland, Kristen M. Kelly, and Anthony J. Durkin "Method using in vivo quantitative spectroscopy to guide design and optimization of low-cost, compact clinical imaging devices: emulation and evaluation of multispectral imaging systems," Journal of Biomedical Optics 23(4), 046002 (9 April 2018). https://doi.org/10.1117/1.JBO.23.4.046002
Received: 12 September 2017; Accepted: 16 March 2018; Published: 9 April 2018
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Cited by 13 scholarly publications.
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KEYWORDS
Imaging systems
Absorption
Multispectral imaging
Chromophores
Tissue optics
Tissues
Scattering
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