Noncontact speckle contrast diffuse correlation tomography of blood flow distributions in tissues with arbitrary geometries

Siavash Mazdeyasna; Chong Huang; Mingjun Zhao; Nneamaka B. Agochukwu; Ahmed A. Bahrani; Lesley Wong; Guoqiang Yu

doi:10.1117/1.JBO.23.9.096005

24 September 2018 Noncontact speckle contrast diffuse correlation tomography of blood flow distributions in tissues with arbitrary geometries

Siavash Mazdeyasna, Chong Huang, Mingjun Zhao, Nneamaka B. Agochukwu, Ahmed A. Bahrani, Lesley Wong, Guoqiang Yu

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Journal of Biomedical Optics, Vol. 23, Issue 9, 096005 (September 2018). https://doi.org/10.1117/1.JBO.23.9.096005

Abstract

A noncontact electron multiplying charge-coupled-device (EMCCD)-based speckle contrast diffuse correlation tomography (scDCT) technology has been recently developed in our laboratory, allowing for noninvasive three-dimensional measurement of tissue blood flow distributions. One major remaining constraint in the scDCT is the assumption of a semi-infinite tissue volume with a flat surface, which affects the image reconstruction accuracy for tissues with irregular geometries. An advanced photometric stereo technique (PST) was integrated into the scDCT system to obtain the surface geometry in real time for image reconstruction. Computer simulations demonstrated that a priori knowledge of tissue surface geometry is crucial for precisely reconstructing the anomaly with blood flow contrast. Importantly, the innovative integration design with one single-EMCCD camera for both PST and scDCT data collection obviates the need for offline alignment of sources and detectors on the tissue boundary. The in vivo imaging capability of the updated scDCT is demonstrated by imaging dynamic changes in forearm blood flow distribution during a cuff-occlusion procedure. The feasibility and safety in clinical use are evidenced by intraoperative imaging of mastectomy skin flaps and comparison with fluorescence angiography.

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Siavash Mazdeyasna, Chong Huang, Mingjun Zhao, Nneamaka B. Agochukwu, Ahmed A. Bahrani, Lesley Wong, and Guoqiang Yu "Noncontact speckle contrast diffuse correlation tomography of blood flow distributions in tissues with arbitrary geometries," Journal of Biomedical Optics 23(9), 096005 (24 September 2018). https://doi.org/10.1117/1.JBO.23.9.096005

Received: 13 June 2018; Accepted: 4 September 2018; Published: 24 September 2018

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