Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning

Thomas D. Wang; Christopher H. Contag; Michael J. Mandella; Ning Y. Chan; Gordon S. Kino

doi:10.1117/1.1760760

1 July 2004 Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning

Thomas D. Wang, Christopher H. Contag, Michael J. Mandella, Ning Y. Chan, Gordon S. Kino

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Journal of Biomedical Optics, Vol. 9, Issue 4, (July 2004). https://doi.org/10.1117/1.1760760

Abstract

We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located distal to the lenses rotates along the orthogonal axes to produce arc-surface images over a large field of view (FOV). With fiber optic coupling, this microscope can potentially be scaled down to millimeter dimensions via microelectromechanical systems (MEMS) technology. We demonstrate a benchtop prototype with a spatial resolution ≤4.4 μm that collects fluorescence images with a high SNR and a good contrast ratio from specimens expressing GFP. Furthermore, the scanning mechanism produces only small differences in aberrations over the image FOV. These results demonstrate proof of concept of the dual-axis confocal architecture for in vivo molecular and cellular imaging.

©(2004) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Thomas D. Wang, Christopher H. Contag, Michael J. Mandella, Ning Y. Chan, and Gordon S. Kino "Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning," Journal of Biomedical Optics 9(4), (1 July 2004). https://doi.org/10.1117/1.1760760

Published: 1 July 2004

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